Arenoviruses in Vero Cells: Ultrastructural Studies

ultrastructural studies of osteoblast cells in osteopetrotic op/op mouse

introduction: the osteopetrosis is a common disease in man and animals which is characterized by calcium accumulation in bones. the purpose of this study is to eveluate the osteoblast ultrastructure in osteoptrotic op/op mouse. material and methods: in this study 5 op/op (experimental) and 5 control mouse were killed by overdose of choloroform. their femur bones were taken, decalcified by edta,...

Morphological development of mumps virus in vero cells

Ultrastructural studies relating to the surface morphology of cultured cells.

Scanning electron microscopy and a surface-replica technique for transmission electron microscopy have been used to study the ultrastructural features of cultured-cell surfaces The presence of microvilh measuring 0-1-0-2 fim in diameter by up to 5 ftm in length has been noted as a regular feature of Landschutz ascites, ' fibroblastic' HeLa, and canine kidney, cells. The surfaces of chick mesenc...

Studies on Rubella Virus: Cytopathic Effect in Vero Cells-cercopithecus Kidney Line

中に この抗体 が明 らかな 変動 を示 さなかつ た, HVJと の混同 は他の血清学的, 生物学的試験成績 か らも明 らか に否定で きた. 次 に血球凝集抑制試験 (HI) による特異 性の証拠 であるが, RV接 種後CPEを 呈す るVero細 胞培養に産生 され るガチ ョウ血球 に 対す る 血球凝集素 (HA) を用 いて, 回復期患者血清, 他 の研究室 由来 のRV 陽性対照血清お よび上記 のRVウ サギ抗血清, 異種 ウ イルス抗血清につ いてHIを 行 ない, 常法 であるBHK21細 胞産生 のRVのHAを 用 いた成績 と比較 した とこ ろ本 質的差 のない成績 を得た. す なわ ち, CPEを 起 し てHAを 産 生 してい るVero細 胞培養 でRVが 増殖 しているこ とを示 唆 している. 以上 に加 えて, 1) RV感 染細胞 にヘ...

Ultrastructural studies on mitochondrial swelling.

Mitochondrial swelling induced by valinomycin, calcium chloride and P(i) was studied after potassium permanganate fixation in suspension. Valinomycin induces a rapid K(+) influx, increase of the matrix space and out-folding of the cristae, with good preservation of the matrix material. This swelling is reversible but the cristae do not completely re-form and have a blebbed appearance. On repeat...